Drop a sequence into the input field in FASTA format. You can view an exon intron map by entering exons in uppercase and introns in lowercase. Set the primer design parameters to whatever makes sense for your experiment (default guidelines are provided) and choose which output files you would like to see
Visualize the location of all designed primers along the sequence by choosing the 'Primer sequence map'. Other output files provide comprehensive visual guides to the designed primers as well as the provided input sequence. Draw your own primers in specific locations by selecting the 'Primer drawing canvas'